葛微课题组在《Neurobiology of Aging》 杂志发表研究论文“Quantitative protein profiling of hippocampus during human aging （2016）”。

在探究衰老过程中，人脑海马区蛋白的动态变化及参与的相关信号通路，中国医学科学院基础医学研究所，“医学分子生物学国家重点实验室”的葛微课题组通过研究发现衰老过程中，人脑海马区具有结合和催化活性的蛋白表达，ETC和突出囊泡融合信号通路发生显著变化

      【摘要】：The hippocampus appears commonly affected by aging and various neurologic disorders in humans, whereas little is known about age-related change in overall protein expression in this brain structure. Using the 4-plex tandem mass tag labeling, we carried out a quantitative proteomic study of the hippocampus during normal aging using postmortem brains from Chinese subjects. Hippocampal samples from 16 subjects died of non-neurological/psychiatric diseases were divided into 4 age groups: 22-49, 50-69, 70 -89, and>90. Among 4582 proteins analyzed, 35 proteins were significantly elevated, whereas 25 proteins were downregulated, along with increasing age. Several upregulated proteins, including transgelin, vimentin, myosin regulatory light polypeptide 9, and calcyphosin, were further verified by quantitative Western blot analysis of hippocampal tissues from additional normal subjects. Bioinformatic analysis showed that the upregulated and downregulated proteins were largely involved in several important protein-protein interaction networks. Proteins in the electron transport chain and synaptic vesicle fusion pathway were consistently downregulated with aging, whereas proteins associated with Alzheimer’s disease showed little change. Our study demonstrates substantial protein profile changes in the human hippocampus during aging, which could be of relevance to age-related loss of hippocampal functions.

      相关结果已发表于《Neurobiology of Aging》 杂志（2016，39：46-56），其作者为葛微课题组博士研究生许本洪，重点室葛微，马超教授是本文的通讯作者。该研究工作得到了以下基金的支持：National Natural Science Foundation of China (81373150, 81271239, 81202371, and 81171091), the CAMS Neuroscience Center Special Fund (#2014C01), and National High Technology Research and Development Program (“863” Program) of China (2013AA020106).。