葛微课题组在《Experimental Gerontology》 杂志发表研究论文“Temporal lobe in human aging: A quantitative protein profiling study of samples from Chinese Human Brain Bank （2016）”。

      在探究衰老过程中，人脑颞叶区蛋白的动态变化及参与的相关信号通路，中国医学科学院基础医学研究所，“医学分子生物学国家重点实验室”的葛微课题组通过研究发现衰老过程中，神经元退化过程中，突出信号传导减少，髓鞘损伤，最终引起细胞结构不稳定。

      【摘要】：The temporal lobe is a portion of the cerebral cortex with critical functionality. The age-related protein profile changes in the human temporal lobe have not been previously studied. This 4-plex tandem mass tag labeled proteomic study was performed on samples of temporal lobe from Chinese donors. Tissue samples were assigned to four age groups: Group A (the young, age: 34±13 years); Group B (the elderly, 62±5 years); Group C (the aged, 84± 4 years) and Group D (the old, 95± 1 years). Pooled samples from the different groups were subjected to proteomics and bioinformatics analysis to identify age-related changes in protein expression and associated pathways.Weisolated 5072 proteins, and found that 67 proteinswere downregulated and 109 proteinswere upregulated in one or more groups during the aging process. Western blotting assays were performed to verify the proteomic results. Bioinformatic analysis identified proteins involved in neuronal degeneration, including proteins involved in neuronal firing, myelin sheath damage, and cell structure stability. We also observed the accumulation of extracellular matrix and lysosomal proteins which imply the occurrence of fibrosis and autophagy. Our results suggest a series of changes across awide range of proteins in the human temporal lobe thatmay relate to aging and age-related neurodegenerative disorders.

      相关结果已发表于《Experimental Gerontology》 杂志（2016，73：31-41），其作者为葛微课题组博士研究生许本洪，重点室葛微，马超教授是本文的通讯作者。该研究工作得到了以下基金的支持：National Natural Science Foundation of China (81373150, 81271239), the CAMS Neuroscience Center Special Fund (#2014C01), and National High Technology Research and Development Program (“863” Program) of China (2013AA020106)。