彭小忠课题组课题组在《Oncotarget》 杂志发表研究论文“Interplay between PCBP2 and miRNA modulates ARHGDIA expression and function in glioma migration and invasion （2016）”。

      本文研究RNA结合蛋白PCBP2调节Rho GDP相关抑制剂ARHGDIA的表达，最终影响神经胶质瘤的迁移和侵袭的机制。中国医学科学院基础医学研究所，“医学分子生物学国家重点实验室”的彭小忠课题组课题组发现RNA结合蛋白PCBP2利用miR-151-5p和miR-16减弱其靶基因ARHGDIA的功能，从而促进神经胶质瘤的迁移和侵袭。

      【摘要】：RNA-RNA and protein-RNA interactions are essential for post-transcriptionalregulationin normal development and may be deregulated in cancerinitiation and progression. The RNA-binding protein PCBP2, an oncogenic protein in human malignant gliomas, is an essential regulator of mRNA and miRNA biogenesis, stability and activity.Here, we identified Rho GDP dissociation inhibitor α (ARHGDIA) as a target mRNA that binds to PCBP2, and we uncovered the role of ARHGDIA as a putative metastasis suppressor through analyses of in vitro and in vivo models of EMT and metastasis. Furthermore, we demonstrated that ARHGDIA is a potential target of miR-151-5p and miR-16 in gliomas. The interaction between PCBP2 and the 3’UTR of the ARHGDIA mRNA may induce a local change in RNA structure that favors subsequent binding of miR-151-5p and miR-16, thus leading to the suppression of ARHGDIA expression. PCBP2 may facilitate miR-151-5p and miR-16 promotion of glioma cell migration and invasion through mitigating the function of ARHGDIA.

      相关结果已发表于《Oncotarget》 杂志（2016，ePublished），其作者为彭小忠课题组课题组博士研究生林细华，重点室彭小忠研究员是本文的通讯作者。该研究工作得到了以下基金的支持：973、国家自然基金。